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Yeast transformants were grown in rich yeast-extract peptone dextrose (YPD) medium (10 g/L yeast extract, 20 g/L bacto-peptone, 20 g/L glucose) supplemented with or without 0.5 g/mL aureobasidin A (Takara Bio, Shiga, Japan), and in selective synthetic dextrose (SD) medium (6.7 g/L yeast nitrogen base without amino acids, 20 g/L glucose) supplemented with appropriate concentrations of amino acids and nucleobases

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Many natural and synthetic substrates used by laccases have an ortho -methoxy substituent on the phenol, an electron-donating moiety that facilitates oxidation 29

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