Organs were homogenized in PBS using a Mixer Mill MM400 (Retsch) with 3.2-mm stainless steel beads (BioSpec) at 30 Hz for 2 min
Gallbladder events : As with other incretin-based therapies, there is a potential risk of gallbladder disease, including cholelithiasis (gallstones)
In a large cohort study, nearly all cases of initial thyrotoxicosis evolved late hypothyroidism, with overt thyrotoxicosis occurring a median of 5 weeks after the first ICI dose and overt hypothyroidism developing a median of 14 weeks later (Muir et al
Chromatography was performed on a 2.1 150 mm, 1.7 m, BEH C18 column (Waters) at 45 C using aqueous HCOOH (0.1% in H 2 O) as solvent A and MeCN containing 0.1% HCOOH as solvent B and the following gradient (flow rate: 0.4 mL/min): For dipeptide analysis in the hydrolysates, chromatography started with 1% solvent B for 2.5 min, which was increased to 95% within 5.5 min, hold for 1.5 min and, then, decreased again to 1% within 0.5 min
Renal IRI or sham surgery was performed prior to the glucose tracing experiment
