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Plasma GHK declines roughly 2.5-fold from age 20 (~200 ng/mL) to age 60 (~80 ng/mL) [2] , coinciding with the age-related loss of regenerative capacity that motivates the exogenous-GHK-Cu work that followed

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Cells were passaged at confluence with 0.05% trypsin and maintained in a 37 C incubator with humidified atmosphere of 5% CO 2 and 95% air

Topically applied copper peptides, in concentrations up to 4%, are generally considered safe and well tolerated by all skin types