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Similar effects with elevated antioxidant markers and decreased ER stress markers were detected after treatment with N-acetlycysteine in cultured murine corneal endothelial cells with FECD [172]

The simultaneous interaction with the endogenous Ub-E2-E3 complex and the protein of interest results in the generation of an Ub-E2-E3-neosubstrate complex, ultimately leading to ubiquitinylation and degradation of target protein

Whereas the rate constant k is determined by using data collected within a specific time frame rather than a discrete instance of time, it is hence a more robust method of analysis

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