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Oligomeric peroxiredoxin-I is an essential intermediate for p53 to activate MST1 kinase and apoptosis

Merilinen S, Mkel J, Sormunen R, Alaoja Jensen H, Rimpilinen R, Vakkala M, Rimpilinen J, Ohtonen P, Koskenkari J, Koivukangas V, Karttunen T, Lehenkari P, Hassinen I, Juvonen T

What actually decides whether a peptide is appropriate Clinically, the compound is rarely the first question I ask

For intracellular cytokine, staining cells were fixed with BD Cytofix Cytoperm (BD Biosciences) for 20 min at 4 C, washed twice with 1x saponin buffer (0.5% (w/v) saponin [Carl Roth], 2 mM EDTA, 2% FCS, in PBS) and stained for IFN (see Table 1) overnight at 4 C in 1 saponin buffer

Krimpenfort P, Berns A (March 2017)