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Among reactive oxygen species, the superoxide anion (O) is primarily generated as a by-product of mitochondrial oxidative phosphorylation when electrons leak from the electron transport chain

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However, absence of identified source and standardized extraction and purification protocols have remarkably influenced the application of polysaccharides

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Ion-exchange chromatography is particularly effective for separating charged species, while size-exclusion chromatography can remove aggregates or degradation products