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Moreover, the authors performed q RT-PCR to quantify Nrf2 mRNA expression, demonstrating that quercetin upregulated this key antioxidant transcription factor while restoring endogenous antioxidant enzyme activity and reducing tissue damage (Sharma et al., 2020

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Ghosh, I

Determination of Michaelis-Menten constant for monobromobimane For validation of the assay, the enzymatic reaction between monobromobimane and glutathione was characterised to determine kinetic parameters such as apparent Michaelis-Menten constant and maximum rate of the reaction

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