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Accordingly, basic FGF (bFGF) (EssexBio, Zhuhai, Peoples Republic of China) dissolved in saline was used

Meanwhile, immunofluorescence (Figure 6B) results revealed that anwuligan treatment promoted the nuclear localization of Ctnnb1 in HFSCs, and this effect was inhibited by the Wnt pathway inhibitor XAV939

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Reducing agent TCEP (5 mM) and alkylating agent IAA (10 mM) were added, followed by sonication and incubation at room temperature in the dark for 30 min each