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Viral infection For lentiviral production, HEK293T cells were co-transfected with 4 g 8.91, 1 g pVSVG, 5 g of lentivector (shRNA targeting SHMT2 : CCAGAATTTGTAGCTGAGATT, and ATF4 : GCGAGTGTAAGGAGCTAGAAA, obtained from RNA Technology platform and Gene manipulating core, Academia Sinica) by using 30 L of Turbofect (Thermo, R0533) in 1 mL of Opti-MEM (Gibco, 31985070) according to the manufacturers instructions

doi: 10.2353/ajpath.2010.090617 28 MariuzziL.DomenisR.OrsariaM.MarzinottoS.LonderoA

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These results concur with the findings of Zia et al

Discussion Collectively, these results suggested that ACTL6A, which contains a hydrophobic region, cooperates with NRF2 to regulate GCLC expression at the transcriptional level and then promotes GSH synthesis and inhibits the ferroptosis of GC cells