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The supernatant was collected and stored in an Eppendorf tube at 80C until analysis

ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States) at 30 min ligation time

The anti-calcific effect of zinc on human valve interstitial cells (hVIC) calcification is, at least in part, mediated through the inhibition of apoptosis and osteogenic differentiation via the GPR39-dependent ERK1/2 signaling pathway

Nutritional Profile: High in healthy fats, fiber, and also in vitamins C and E