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Mitochondrial energy metabolism dysfunction involved in reproductive toxicity of mice caused by endosulfan and protective effects of vitamin E
The nitrocellulose membranes were immunoblotted with primary antibodies against acyl-CoA synthetase long-chain family member 4 (ACSL4) (1:1000, Santa cruz), Bax (1:1000, Cell signaling), Bcl-2 (1:1000, Cell signaling), DRP1 (1:1000, Cell signaling), GAPDH (1:5000 Abcam), GPX4 (1:1000, Abcam), MLKL (1:1000, Invitrogen), OPA1 (1:1000, Cell signaling), pDRP1 (Ser616) (1:1000, Cell signaling), pRIPK3 (Ser232) (1:1000, Cell signaling), and RIPK3 (1:1000, Cell signaling) overnight at 4 C, and then incubated with the appropriate secondary antibody (1:1000, Santa cruz)
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Decreased expression of antioxidant and key mitochondrial genes were also detected (74)
