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Successive 5-m lung sections were placed on slides and subjected to HE and MT staining, according to previously described methods, with minor modifications ( Western Blot Analysis Lung tissues were homogenized in ice-cold radioimmuno precipitation (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Haimen, China)

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(ii) GSH nonenzymatically reacts with the disulfide substrate, yielding a GSSR substrate that is reduced as described above 9,46,57

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