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The primers used were 5-caccatcacctcctggaaga-3 and 5-gggtgcagaatctcgagttg-3 for Pck1 , 5-gtggcagtggtcggagact-3 and 5-acgggcgttgtccaaac-3 for G6pc , 5-tacagagtgctggccaagag-3 and 5-ttcaaggatgcactggagag-3 for Cyp7a1 , 5-tagccctctttcctccactcata-3 and 5-gaagcgatcgaacctaaattcct-3 for Cyp7b1 , 5-gcctcacctatgggatcttca-3v and 5v-tcaaagcctgacgcagatg-3v for Cyp27a1 , 5-ggctggcttcctgagcttatt-3 and 5-acttcctgaacagctcatcgg-3 for Cyp8b1 , 5-acctgtctaacctcttcacc-3 and 5-caatgctgaggttcatgtcc-3 for Slc10a1 , 5-ggaagattgttggcccgatt-3 and 5-agtgggagttatggtcaggt-3v for Slco1a1 , 5-gcgtcgtgattagcgatga-3 and 5-atggcctcccatctcctt-3 for Hprt
The health risks are too high to be negligent
I would recommend her 100x over
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While the bac water might prevent bacterial growth for a short period, many peptides degrade rapidly at room temperature
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