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Proteins were fractionated electrophoretically on NuPAGETM 412% Bis-Tris Polyacrylamide Gel (Invitrogen) and then transferred to a polyvinylidene fluoride (PVDF) membrane using the iBlot Dry Blotting System (Invitrogen)

The results may vary depending on the type of peptide administered and the effects sought

doi:10.1371/journal.pone.0093315 Yang Y, Jin X, Jiang C (2014) S-glutathionylation of ion channels: insights into the regulation of channel functions, thiol modification crosstalk, and mechanosensing

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