Pre-proLOX undergoes post-translational modifications within the ER and, then, experiences N-glycosylation of the N-terminal pro-peptide chain (147 aa residues) and folding of the C-terminal (containing the mature protein, 249 aa residues) to form three or more disulfide bonds (Trackman et al ., 1992
Check blood pressure (hypotension can cause compensatory HR increase)
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When you're investigating something as delicate as meniscus regeneration, you need to be absolutely certain that the molecule you're studying is precisely the molecule it's supposed to be