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2.5.2 LCMS/MS data-dependent acquisition Digested samples were analyzed by nano HPLC using an EASY-nLC 1,000 chromatograph coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific, Pittsburgh, PA, United States), using an EASY-Spray Accucore (P/N ES801) Thermo Scientific reverse phase column coupled to an EASY-SPRAY Electro Spray ionizer (Thermo Fisher Scientific, Pittsburgh, PA, United States), with Spray voltage between 2.53.5 kV

Long-Term Maintenance Planning As you approach your goal weight, your provider works with you to develop a sustainable maintenance strategy that helps you keep the weight off long-term including ongoing medication management if appropriate and lifestyle optimization

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