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Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue

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Subsequently, in the second reaction catalyzed by the enzyme glutathione synthetase (GS), GSH is produced from -glutamylcysteine and glycine [36]

If there is one place where the thymosin beta-4 story is most clearly regenerative in a specific, mechanistic sense, it is myelin