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Materials and methods Animals Wistar albino male rats (aged 12 weeks, 350400 g b.w.) were bred in-house (the animal facility at the Department of Pharmacology, School of Medicine, Zagreb, Croatia

Reactive oxygen species formation and its effect on CD4+ T cell-mediated inflammation

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We also extracted RNA from five of the plants that we genotyped to be homozygous and performed reverse transcription to make cDNA, which will serve as proof that the mutant plants are incapable of expressing the Coi gene