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Mechanisms investigated Metabolic-pathway research converges on a small set of enzymatic and receptor targets, and these compounds map onto different ones they are not interchangeable: NNMT inhibition (5-Amino-1MQ): studied as a small-molecule inhibitor of nicotinamide N-methyltransferase, an enzyme implicated in cellular NAD/SAM metabolism

Gene expression analysis RNA sequencing Liver tissue and skeletal muscle tissue (quadriceps) (~0.5 cm 3 in each case) were extracted immediately after rats were euthanized, placed into Eppendorf tubes with RNA (Invitrogen, catalogue no

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