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The left ventricle was perfused intra-aortically with 250 ml of ice-cold, 0.1 M buffer phosphate [containing (mM): 137 NaCl, 2.7 KCl, 10 Na 2 HPO 4 , and 2 KH 2 PO 4 ] with heparin (10000 U/L), pH 7.4, at a flow rate of 1825 ml/min, followed by 4% paraformaldehyde +12% picric acid solution until transparent liquid flowed from the right ventricle

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Trend or condition retesting used when the water source is variable (private well, seasonal changes), used after events (flooding, plumbing work, main break), used after maintenance (well disinfection, repairs) to verify the condition improved

Journal of Applied Physiology, 110(3), 774780