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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain

P., Kesslak, J

Imaizumi Y, Murota H, Kanda S, Hishikawa Y, Koji T, Taguchi T, Tanaka Y, Yamada Y, Ikeda S, Kohno T, et al

In a recent small randomized controlled trial, dietary supplementation with methylation-modifying leucovorin/folate improved core symptoms of ASD

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