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Sample volumes were normalized to 35 L with EPPS buffer (200 mM, pH 8), diluted with HPLC grade CH 3 CN (9 L), and then labeled (5 L of 20 g/L per sample) with the corresponding TMTsixplex Isobaric Mass Tag (Themo Scientific, #90064B)

Mouse studies suggest biotransformation of glucoraphanin to SFN occurs in the colon (21, 25, 26), and we previously confirmed the majority of SFN accumulation takes place in the colon, with greater resolution using multiple locations along the GI tract (13)

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