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Use a fuse puller to remove the fuses, starting with the ones with the smallest amp ratings and working toward the ones with the highest amp ratings

At the end of this incubation period, the cultures were centrifuged 10 min at 13,000 rpm, the supernatants were discarded, and each pellet was suspended in 0.6 ml of lysis buffer (1 M glycerol, 0.1 M EDTA)

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This makes it a lighter option for those who enjoy a gentler smoking experience

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