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For IF staining, fixed with 4% PFA for 15 min, permeabilized with 0.3% Triton X-100 in PBS for 10 min, and blocked with 5% normal donkey serum for 1 h at room temperature (RT)

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If you restrict most or all of these wonderful vitamin B12 sources from your diet, you are at a high risk of vitamin B12 deficiency