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The cells were incubated with 1:50 solution of primary antibodies, for p -FAK and YAP, overnight at 4C, and then incubated with secondary antibodies and Hoechst 33,342 (Thermo Fisher Scientific) at RT for 30 min

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In one embodiment, the buffer is a sodium bicarbonate buffer having a pH of about 7.5 to about 8.5

Enhanced Daytime Functioning: Patients experience better memory retention, mood stabilization, and cognitive clarity due to the regenerative effects of stem cells on the hippocampus and prefrontal cortex