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Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]

We have found a way to increase the solubility of niclosamide and therefore its bioavailability to infectable and infected epithelial cells just by slightly increasing the pH in simple aqueous buffered solutions (48)