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Splitting vials across titration phases Some researchers reconstitute their 10 mg vial at a higher concentration (5 mg/mL with 2 mL water) for the later phases when precision is less critical and injection volume matters more

10.1021/cr800404j Chem Rev

The most common reconstitution mistakes involve rushing this step

However, this approach is known to lead to partial loss of the native phenotype of these cells, particularly when the cells are cultured in matrix-free cultures, as described by von der Mark et al