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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts
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Guthrie L, Spencer SP, Perelman D, Van Treuren W, Han S, Yu FB
however, the incidence of acute gallbladder disease was greater in semaglutide-treated patients than in placebo-treated patients, even after accounting for the degree of weight loss
PMID: 31735792 5 Megaloblastic Anemias: Nutritional and Other Causes
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