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A key innovation in this field is the development of designer probioticsgenetically engineered or otherwise modified commensal bacterial strains specifically tailored to withstand production stresses such as lyophilization, thermal fluctuations, and gastric acidity, or to enhance their most beneficial functions for the host (537)

Subsequently, the medium was replaced with OPTI-MEM supplemented with 5% FBS and 1% sodium pyruvate

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