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Immunostaining revealed mixed cell populations with many undifferentiated hPSCs and mesoderm cells, and DE markers SOX17 and FOXA2 were expressed at lower levels compared to the Activin A-based control (CA)

Treatment failures have been reported if acetylcysteine was stopped prematurely

Primary sites: Abdomen (avoiding 2 inches around the navel) Outer thighs Back of upper arms Upper buttocks Rotation pattern example: Day 1: Left abdomen Day 2: Right abdomen Day 3: Left thigh Day 4: Right thigh Day 5: Left arm Day 6: Right arm Day 7: Return to left abdomen Keep at least one inch between injection sites in the same general area

To optimize the number of molecules presented in the networks, the cut-off value for correlation was 0.95 for integrating three or more datasets, or 0.7 for integrating two datasets

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