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After 24 h, treated biofilms were washed once with 1 PBS followed by addition of 200 L of 1 PBS and 15 L of a 0.05% w/v resazurin solution

[4] Water with 2 different temperatures (warm and cool) that flow alternately, diluted Chinese herbal medicine, and diluted coffee are used as enema solution for colon irrigation, retention enema, and coffee enema, respectively