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C) Assessment of the impact of exogenous glutathione application on charcoal rot disease severity i) Establishment and maintenance of plants A greenhouse experiment was conducted with two charcoal-rot-resistant (SC599, SC35) and two susceptible (Tx7000, BTx3042) sorghum lines
cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)
47 HuT.LuF.ZhangH.YuanZ.HeP
d , OGT , PRPS1 , and PRPS2 mRNA levels in normal adjacent tissue (N) and tumor tissue (T) from lung cancer patients
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