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Iodoacetic Acid (IAA) Neurotoxicity Assays HT-22 cells were seeded into 96-well flat-bottomed plates at a density of 3000 cells/well in 100 l DMEM (high glucose, 1 mM pyruvate, 10% FBS) and allowed to attach overnight

Maintain adequate protein intake (80-100g daily), take biotin supplement (5,000mcg), and ensure youre getting enough iron and zinc to minimize hair loss risk

Very high doses can result in psychosis, which rarely occurs at therapeutic doses even during long-term use

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The theory is that hangovers occur because of a build-up of acetaldehyde in the body