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Upregulation of -GCS-HS mRNA in Mucosa from TNBS-Treated Rats To examine if the partial recovery of the mucosal GSH levels observed between 12 weeks after TNBS+ethanol treatment was due to an upregulation of the rate limiting enzyme in the biosynthesis of GSH, we analyzed the -GCS-HS mRNA levels in mucosa from TNBS-treated rats
Primary antibodies used were GAPDH (Sigma-Aldrich, #G8795, diluted 1:10,000), AKAP1 (Sigma-Aldrich, #SAB4503247, diluted 1:500), PHACTR1 (Abcam, #ab229120), Total OXPHOS Blue Native WB Antibody Cocktail (Abcam, #ab110412), HMOX1 (Santa Cruz Biotechnology, #sc-7695) and the following all from Cell Signaling: CDT1 (#8064), Thymidine Kinase 1 (#28755), Phospho-Histone H3 (Ser10, #53348), Cyclin A2 (#91500), Cyclin B1 (#12231), Cyclin E1 (#20808), Phospho-cdc2 (Tyr15, #4539), Tom20 (#42406), OPA1 (#80471), DRP1 (#8570), Phospho-DRP1 (Ser616, #4494), Phospho-DRP1 (Ser637, #6319), MFF (#84580), Mitofusin-1 (#14739), Mitofusin-2 (#11925), GPX4 (#52455), NCOA4 (#66849), KEAP1 (#8047), FTH1 (#4393) and 4F2hc/SLC3A2 (#47213)
Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells
Recognizing these helps ensure appropriate responses
