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Experimental model and subject detail Cell lines The human lung adenocarcinoma cell line H838 (gender: male) was purchased from ATCC (CRL-5844) and cultivated in Dulbeccos modified Eagles Medium (DMEM, Lonza) supplemented with 10% fetal calf serum (Gibco), 100 g/ml streptomycin (Gibco) and 100 U/ml penicillin (Gibco) at 37C in a humidified atmosphere with 5% CO 2

Silent information regulator 1 (SIRT1), an NAD + -dependent deacetylase, functions as a central regulator of metabolic homeostasis and a key mediator in immune microenvironment remodeling and inter-organ communication

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Oral chelation may be done at home in some cases, but intravenous chelation should always be performed in a medical setting under professional supervision to ensure safety and proper monitoring