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Reducing intestinal barrier permeability attenuates arthritis Since our results suggested that increased zonulin levels along with impaired intestinal permeability for lactulose and FITC-Dextran (FD4) are preceding the onset of murine and human arthritis, we sought an intervention that strengthens the intestinal barrier and may disrupt the transition from autoimmunity to inflammation and thereby inhibit the onset of arthritis

The bioregulator peptide approach of modulating existing biological pathways rather than forcing artificial responses distinguishes PTD-DBM from many conventional treatments

The cell line was grown and maintained by culturing with feeder cells (SL10, Reprocell Inc.) at 37 C in 5% CO 2 in a humidified atmosphere, using Primate ES cell medium (Reprocell Inc.), supplemented with bFGF (Fujifilm Wako Pure Chemical Corporation)

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Twice-daily dosing during the first 1-2 weeks followed by once-daily dosing is a commonly described approach in the research community