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Mistry HD, Gill CA, Kurlak LO, Seed PT, Hesketh JE, Mplan C, Schomburg L, Chappell LC, Morgan L

The activity of NRF2 is highly dependent on several signaling pathways, including protein kinase RNAlike endoplasmic reticulum kinase (PERK) pathway and mitogenactivated protein kinase (MAPK) pathway, making it a complex and contextdependent transcription factor [79, 80]

Panels A, B, and C show the percentage of surviving hepatocytes for a 15 g dose, for an 18 g dose, and for a 30 g dose for males and females, respectively

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Cells were washed and incubated with Cy-3 or Cy-5 conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA and Thermofisher)