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Hippocampi from P4 Sprague Dawfey rats were cultured as previously described (Wayman, Impey et al

Since the tissue retains excess water after staining, dehydrate the sections by immersing them in absolute ethanol for 1 min

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Understanding the roles and interventions of the GSH system, as well as its connection with ferroptosis, may favor the advancement of more effective therapies to prevent myocardial injury (summarized in Fig