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For long-term studies or to prevent degradation from repeated freeze-thaw cycles, it is best practice to aliquot the freshly reconstituted solution into single-use volumes and store them frozen at -20C or colder

NAD+ is a coenzyme every cell uses to produce energy and run repair and metabolism

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When a cell over-expresses NNMT, it does two costly things at once: it burns through methyl-donor capacity, and it diverts nicotinamide away from NAD+ regeneration into an inert metabolite (1-methylnicotinamide) that gets excreted

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