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however, its deubiquitylation specificity toward long or complex ubiquitin chains has not been well-characterized

Following incubation, cells were centrifuged at 1000 rpm for 3 min at 4 C, washed twice with cold PBS to remove excess dye, and resuspended in 1 mL of serum-free medium (or PBS)

Overall, the literature suggests that aging is associated with increased OS in brain and body, but the timing and regional distribution of changes requires further study