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The organic layers from two extractions were combined and aliquots were analyzed on a 4000 Q-Trap mass spectrometer (AB SCIEX, Framingham, MA) using the 50 2.1 mm Hydrophilic Interaction Liquid Chromatography (HILIC-LC) column (Waters Corp., Milford, MA)

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With GSH depleted, IKK becomes constitutively active, driving continuous transcription of TNF-, IL-6, and IL-1